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KMID : 0377619630040010061
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Korean Jungang Medical Journal
1963 Volume.4 No. 1 p.61 ~ p.72
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Effect of Heat on Hemagglutinin
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Abstract
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In the previous paper, the author reported that an anti-goat-cell serum or an antichicken-cell serum heated at a certain temperature (72°C and 76°C) showed higher hemagglutination titer than when heated at lower temperatures, and also prolonged heating increased hemagglutinating activity. This curious phenemenon, refered to as heat-activated, hemagglutination, was not observed with all sera employed, but usually in the sera of the rabbits immunized a comparatively long period. The present investigation was undertaken in order to understand the nature and the mechanisms involved in such a peculiar phenomenon.
Anti-goat-cell sera were obtained as described in the previous paper and the sera showing heat-activated hemagglutination were employed in the present study.
The serum heated to 72°C agglutinated goat as well as sheep red cells, but not human, chicken, horse or cow red cells and the agglutinating activity could be removed by absorption with goat red cells before or after heating at 72°C, but not with guinea-pig red cells. These facts indicate heat-activated hemagglutination is a specific immunological reaction.
Neither heated(72° or 76°C) normal rabbit serum added to heat-activated hemagglutinating serum(inactivated at 56°C 30 minutes), nor inactivated normal rabbit serum added to heated immune serum exerted any influence upon the latter.
Employing trypsin treated red cells and the indirect Coombs test, incomplete antibody was detected in the heat-activated hemagglutinating serum. There was found, however, no relation between heat-activated agglutination and the presence of incomplete antibody. Though the immune serum heated at 72°C or 76°C might show a significantly higher agglutinin titer than when inactiyated at 56°C, the hemolysin titr was lower by far.
Also a fact of interest was observed that the heated immune serum once attached to red cells showed an inhibitory effect upon hemolysis in the presonce of adequate hemolysin and complement subsequently added.
Significant differences in the agglutinating titers were noted according to serum dilution prior to heating. The more serum was diluted, the higher became the hemagglutinating titer.
A heat-activated hemagglutination was observed when the immune serum was diluted with Ringer´ s solution or 0.45% salt solution instead of physiological salt solution, but the serum diluted with saline containing strong acid did not show it.
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